CD BioGlyco, a biotechnology company that offers a full range of glycobiology-related products, analysis, custom synthesis and design for customers all over the world, now supports glycan separation and purification with advanced techniques.
Analysis of protein glycosylation is possible at many levels, including intact glycoproteins, glycopeptides, and liberated glycans. To effectively separate the various glycoforms of glycoproteins, an examination of the entire glycoprotein is challenging, particularly for proteins with a diversity of glycosylation sites. Assigning certain glycan structures to various glycosylation sites is possible using site-specific information from glycopeptide analysis, which may identify the allocation of glycosylation to particular places on proteins. The molecular makeup of proteins is also important to comprehend.
By using chemical or enzymatic techniques, glycans can be freed from proteins, and derivatization processes are then frequently used to enhance the glycans’ characteristics for examination. Additionally, glycan enrichment must be carried out when working with complicated biological materials.
Supported by professional researchers and experimental instruments, CD BioGlyco now provides customers with efficient separation, purification and analysis of glycans. The main techniques provided by CD BioGlyco include:
Capillary Electrophoresis (CE)
Based on CE technology, CD BioGlyco offers a top-notch service for identifying the structure and linkage composition of unidentified glycans in samples.
Reversed Phase Liquid Chromatography (RPLC)
In glycomics, RPLC is a popular and useful LC separation technique. It is compatible with electrospray ionization and offers repeatable results and good separation.
Hydrophilic Interaction Chromatography (HILIC)
A variety of HILIC separation techniques have been developed by CD BioGlyco to assist customers in resolving the separation issues associated with strong polar pharmaceuticals and tiny biomolecules.
Porous Graphitized Carbon (PGC)
Glycans released from complicated samples can be characterized by CD BioGlyco employing PGC-LC-MS.
Lectin Enrichment
Mass spectrometry can be used to enrich and detect glycoproteins expressing particular glycans using lectin enrichment techniques developed by CD BioGlyco.
“With analytical equipment with high accuracy, professional teams dedicated to glycans separation and detection, and service with high flexibility, we are confident to provide customers with characteristic and efficient detection solution.” Said Anna, one of the representative speakers from CD BioGlyco.
To know more detailed information about the glycan separation and purification service provided by CD BioGlyco, please visit https://www.bioglyco.com/glycan-separation-and-purification-service.html.